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Fig. 9. pax6 is not required after stage 12 for other retinal stem cell or neural identity marker genes but is necessary for some differentiation markers. (A) rx1 expression domain in the optic vesicle is smaller (arrow). (B) At stage 38, rx1 expression is similar to controls and no repression surrounds β-Gal-labeled nuclei (arrowheads). (C) six3 expression domain in the optic vesicle is smaller (cf. Fig. 5F). (D) At stage 38, no repression of six3 surrounds β-Gal-labeled nuclei (arrowheads). (E) notch1 expression domain in the optic vesicle (arrow) does not change, and β-Gal labeled cells (red) express notch1 (inset). (F) At stage 38, no repression of notch1 expression surrounds β-Gal labeled cells (arrowheads). (G) sox2 expression domain in the optic vesicle is smaller (arrow). (H) At stage 38, no repression of sox2 expression surrounds β-Gal-labeled nuclei (arrowheads). (I) ngn1 expression in the optic cup (arrow) is fainter (cf. Fig. 5L). (J) At stage 38, ngn1 continues to be repressed surrounding β-Gal labeled nuclei (arrowhead). (K) p27 expression in the optic cup (arrow) is fainter (cf. Fig. 5N). (L) At stage 38, p27 expression continues to be repressed surrounding clusters of β-Gal labeled nuclei (arrowheads). (M) neuroD expression domain in the optic cup (arrow) is reduced in size but not expression intensity (cf. Fig. 5P). (N) At stage 38, neuroD expression is not repressed in β-Gal labeled cells (arrowheads). (O) Xath5 expression domain in optic cup (arrow) is fainter in less than half of the embryos (cf. Fig. 5R). (P) At stage 38, Xath5 expression is not repressed in β-Gal labeled cells (arrowheads). Data for stage 16 activation are similar, but at significantly different frequencies for differentiation genes (see Table 2). Dashed lines indicate the outline of the retinas in the sectioned material. For whole mounts, bars = 100 μm; for sections bars = 20 μm. |
