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notch1xenopus   

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Experiment details for notch1

De Lucchini S et al. (2003) Assay

Expression of 5-HT2B and 5-HT2C receptor genes is associated with proliferative regions of Xenopus developing brain and eye.

Gene Clone Species Stages Anatomy
notch1.L laevis NF stage 40 midbrain , ventricular zone , central nervous system , proliferative region
notch1.L laevis NF stage 45 hindbrain , ventricular zone , central nervous system , proliferative region

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  Fig. 3. Expression pattern of 5-HT2B and 2C receptor mRNAs in the larval brain. The probes used are indicated at bottom of each panel. The figure shows transversal sections of larvae neural tube at stage 40 (A–C) and 45 (D–O). Panels correspond to diencephalon (D–F), mesencephalon (A–C, G–I); anterior (J–L and O) and posterior rhomboencephalon (M–N). Proliferating zones are evidenced by BrdU incorporation (E,H,K) and by labelling with proliferation markers (B,C,N,O). (E,H,K): in blue, Hoechst nuclear staining; BrdU incorporation is viewed as orange fluorescence.

Gene Clone Species Stages Anatomy
notch1.L laevis NF stage 40 retina , ciliary marginal zone , eye
notch1.L laevis NF stage 45 retina , ciliary marginal zone , eye

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  Fig. 4. Expression of 5-HT2B and 2C receptor mRNAs in developing retina. Transverse sections through the eye of a stage 40 (A–C) and 45 larva (D–I). The probes used are indicated at bottom of each panel. The CMZ is labelled by proliferation markers (B,C,E,F) and stained with anti-BrdU antibody after BrdU incorporation (H). In blue, Hoechst nuclear staining; orange, BrdU incorporation. (G) is an enlargement of (D) to show the scattered postmitotic cells expressing 5-HT2B transcripts in the INL. CMZ, ciliary marginal zone; L, lens; INL, inner nuclear layer.