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FIG. 5. Spatial expression of neural-specific genes is unaffected in antisense a6 integrin-treated embryos. Controls and embryos with repressed a6
integrin expression were examined by in situ hybridization for several neural-selective transcripts: nrp1 (A–F), Xanf1 (G–L), and sybII (M–R). In
uninjected, control embryos the expression of these transcripts was examined in embryos of neural plate (stage 13; A, G, and M), neurula (stage 25;
B, H, and N) and tail-bud (stage 35; C, I, and O) stages. The expression of these genes was also examined in embryos injected with antisense a6
integrin constructs and that displayed severe perturbation of neurulation at sibling stages of neural plate (stage 15; D, J, and P), neurula (stage 25;
E, K, and Q), and tail-bud (stage 35; F, L, and R) stages. Scale bar represents 200 mm. |
