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FIG. 1. Binding of MAb-A5 to frozen sections of XWL~JYUS tadpole brains at stage 51. Coronal sections were stained by the ABC method (see
text). (A) MAb-A5-binding to the midbrain. The antibody preferentially binds to the superficial eighth and ninth layers of the tectum (tee), but
not to the deeper parts of the tectum nor tegmentum (teg). (B-E) MAb-A5 binding to the medulla and spinal cord. Arrows indicate MAb-A5
bindings in the medulla at a level just caudal to the vestibulocochlear nerve root (B), at a level close to the obex (C), and in the spinal cord at the
upper cervical level (D) and at the thoracic level (E). Bars, 100 pm |