| |
Fig. 2. B56ε plays a direct role in the regulation of rx expression. (A) RT-PCR showing that maternal depletion of B56ε (oocyte injection) reduced the expression of dorsal specific genes (dickkopf-1, goosecoid, cerberus, and Xenopus nodal related3 (xnr3)) and enhanced the expression of a ventral-specific gene (sizzled) at stage 11. Injection of 7.5 ng of εmor into two dorsal animal blastomeres at the 8-cell stage did not affect the expression of above genes. ODC was used as the loading control. (B) Whole mount in situ hybridization showing that the expression of chd in a control embryo and an embryo injected with 7.5 ng of εmor. (C) Whole mount in situ hybridization showing the expression of rx in a stage 18 uninjected embryo, an embryo injected with 0.5 ng of εmor, an embryo injected with 1 ng of εmor, and an embryo injected with 1.5 ng of εmor. εmor was injected into one of A1 blastomeres at the 32-cell stage. Lineage tracer, n-β-gal, was co-injected with εmor to indicate the side of injection. (D) Whole mount in situ hybridization showing the expression of rx (stage 12.5), sox3 (pan-neural, stage 12.5), otx2 (pan-forebrain, stage 12.5), and pax2 (midbrain, stage 14), arx (diencephalons, stage 14), and eomesodermin (telencephalon, stage 26/27) in control embryos (upper panels) and embryos injected with εmor (4.5 ng, lower panels). The right side was injected as indicated by the Red-gal staining. Embryos were injected unilaterally at the 8-cell stage. |
