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Fig. 4. B56ε is required for IGF1-induced EFTFs expression. (A) Whole mount in situ hybridization showing that εmor injection blocked IGF1-induced rx expression (upper panels), without affecting IGF1-induced otx2 (middle panels) and sox3 (lower panels) expression in whole embryos (stage 14). IGF1 (2 ng) was unilaterally injected at the 8-cell stage into either wild-type embryos, or embryos that were previously injected with 5 ng of εmor. The right side was injected. (B) RT-PCR showing εmor injection blocked IGF1-induced rx, pax6, six3, and lhx2 expression, while IGF1-induced otx2 and sox3 expressions remain unaffected. εmor (10 ng) was injected at the 1-cell stage. IGF1 (2 ng) was radially injected into the animal pole of each blastomere at the 4-cell stage. Animal caps were dissected at late blastula stage and harvested at stage 13. |
