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Figure S8. Expression of Spemann organizer and anterior neural markers during embryogenesis of Xenopus morphants lacking XdnTcf7l2. In situ hybridization was carried out for: the organizer marker chordin (Chd) at st. 10.5 (1st column, vegetal view), the organizer+anterior neuroectodermal marker Otx2 (Otx2) at st. 12.5 (2nd column, anterior up); the anterior neuroectodermal marker Otx2 at st. 20 (3rd column, anterior on); and the midbrain/hindbrain boundary marker engailed 2 (En2) at st. 20 (4th column, anterior on). These hybridizations were carried out in embryos injected with morpholinos in one animal blastomere (left, injected in all panels) of 2-cell stage embryos, with the uninjected side (right in all panels) as a control. (Injected side marked with lacZ.) Morpholinos used were: control (top row); ATG morpholino (middle row), and splicing morpholino (bottom row). Percent tally of results from repeated analyses in multiple embryos (n = 18-46, indicated by white numbers), with respect to level of marker expression on the injected side (same, reduced, or absent), is given in the histograms below each column. Anterior neural development is unaffected by either morpholino prior to st. 12.5, the time at which XdnTcf7l2 mRNA first appears. |
