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FIGURE 5. Unilateral injection of XSMOC-1 antisense morpholino (MO) produces mild ventralization and anophthalmia on the injected side. XSMOC-1 MO (6 ng) was injected into a single blastomere at the two-cell stage. At stage 17 (A and B), mild abnormalities were observed in the developing neural axis of XSMOC-1 MO-injected embryos (B). By stage 32 (C), MO-injected embryos were mildly ventralized (D and E) compared with controls (C). In addition, eyes were absent on the injected side (E); this was more apparent by stage 38 (G). Eye development appeared normal on the noninjected side (F). The XSMOC-1 MO phenotypes shown in D and E were typical for this stage and were observed in 90% of the embryos in five independent experiments (n=164). H, whole mount hybridization in situ analyses of Otx2 (H and I) and Tbx2 (J and K) in stage 32 control (G and J) and XSMOC-1 MO-injected (I and K) embryos. The injected sides are displayed on the right. The arrows indicate the location of the eye fields.
"This research was originally published THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL.284(28) pp.18994-19005, July 10, 2009. Copyright: The American Society for Biochemistry and Molecular Biology." |
