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Fig. 4. The transcription factors en2, XTcf-4 and pax2 regulate each other. A) Injection of 4 pmol en2 specific antisense morpholino oligonucleotide (en2Mo) and a combination of 2 pmol pax2 and 2 pmol pax5 specific antisense morpholino oligonucleotide (pax2/5Mo) into one blastomere of Xenopus 2-cell stage embryos result in reduced XTcf-4 expression at the injected side (asterisk). In either case, normal XTcf-4 expression was restored by coinjection of 200 pg of the corresponding mRNA (en2Mo + en2 mRNA, and pax2/5Mo + pax2 mRNA). B) Quantification of XTcf-4 reduction following en2 and pax knock-down. n: number of analyzed embryos. C) Injection of 4 pmol en2Mo results in reduced pax2 expression, injection of 4 pmol pax2/5Mo results in reduced en2 expression at the injected side (asterisk). In either case, normal expression was restored by coinjection of 200 pg of the corresponding mRNA. D) Quantification of en2 and pax2 reduction following en2 and pax knock-down. n: number of analyzed embryos. E) The depletion of pax2/5 and en2 leads to a reduced wnt-1 expression in tailbud stage embryos, while it does not affect wnt-1 at neurula stage (F). Consistent with the effect seen in tailbud stages the en2 expression is also strongly reduced in early stages (stage 17) after a pax2/5 knock-down. In contrast, the depletion of en2 shows no effect on the pax2 expression in neurula stage embryos. The asterisk marks the injected side. |
