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Fig. S1. Expression of Pax genes is not induced in non-neural ectoderm. (A-C) After grafting neural plates (NP) from stage 13 pigmented donors into the belly ectoderm (B) of stage 13 albino hosts, Pax gene expression was analyzed in tailbud stage embryos. Grafts (outlined) are shown in high magnification views. Expression of Pax6, Pax3 and Pax2 is confined to grafts. (D-H) After grafting belly ectoderm (B) from stage 13 pigmented donors into the lateral neural plate border (NB) of stage 13 albino hosts, expression of Pax genes was analyzed in tailbud stage embryos. None of the Pax genes analyzed is induced in the graft (E is a transverse section through the embryo in D, showing absence of Pax6 in graft; insets in D,H show control sides). |