|
Fig. 4. Effects of mHIP on XPtc1 expression and otic specification. Following injection of mHIP mRNA (750 pg) into one of two blastomeres, embryos were
fixed at stage 38 (A) or stage 36 (B-G) and stained for expression of XPtc1 (A), Xdll-3 (B-E), or XPax-2 (F, G). mHIP-injected embryos (n 24) exhibit
strongly reduced XPtc1 expression (74%; A). In parallel, ectopic vesicular structures (depicted by triangles) expressing Xdll-3 (27%, n 22) and XPax-2
(10%, n 31) were also observed in mHIP-injected embryos. is, injected side; nis, noninjected side. |