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Figure 4. Knockdown of Gli1 or Gli2 rescues phenotypes induced by partial depletion of Dzip1. (A) Whole mount in situ hybridization of ptc1 (top), pax6 (middle) and pax2 (bottom) in controls and embryos either receiving a single injection of Gli1 MO, Gli2 MO, or DMO, and double knockdown embryos injected with Gli1 MO/DMO or Gli2 MO/DMO. In the middle panels, the distance between the floor plate and the ventral border of the pax6 expression domain was highlighted. (B-D) Summary of ptc1 expression phenotypes (B), pax6 expression phenotypes (C) or pax2 expression phenotypes (D) in controls and the different groups of embryos receiving either a single or double morpholino injection against Gli1, Gli2 or Dzip1. |
