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pax3xenopus   

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Experiment details for pax3

Disabled-2: a positive regulator of the early differentiation of myoblasts.

Disabled-2: a positive regulator of the early differentiation of myoblasts.

Gene Clone Species Stages Anatomy
pax3.L laevis NF stage 29 and 30 somite , myotome

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  Fig. 8 Relationship of XDab2L and XDab2S and their spatiotemporal expression patterns. (a) Schematic representation of different domains of XDab2L and XDab2S. (b) Phylogenetic analysis of Dab1 and Dab2 proteins from the frogs, human, mouse, rat, cattle, and fish. (c) qPCR results showing XDab2L and XDab2S expression at different stages of the Xenopus laevis development with ornithine decarboxylase (odc) as the internal control. (d) Expression of XDab2L and XDab2L/XDab2S in Xenopus embryos at different developmental stages after whole-mount in situ hybridization. (e) Vibratome sections showing the expression of XDab2L/XDab2S (i, ii), XMyoD (iii, iv), XPax3 (v, vi), XMef2c (vii, viii), XMyos (ix, x), and XAC100 (xi, xii) after whole-mount in situ hybridization at stage 30. Boundaries of somites are indicated by arrows

Gene Clone Species Stages Anatomy
pax3.L laevis NF stage 31 to NF stage 32 brain , spinal cord , somite , tail bud , hypaxial muscle

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  Fig. 9 Inhibition of XDab2 expression by morpholinos resulted in the downregulation of the expression of myogenic transcription factors in somites. Both blastomeres at the 2-cell stage were injected with control MO (a, c, e, g, i, k) or XDab2 MO (b, d, f, h, j, l), and the injected Xenopus embryos were fixed at stages 31–32 for the examination of the expression of XPax3 (a, b), XMyoD (c, d), XMef2c (e, f), XMyos (g, h), XAC100 (i, j), and XMyf5 (k, l) after whole-mount in situ hybridization

Gene Clone Species Stages Anatomy
pax3.L laevis NF stage 31 to NF stage 32 brain , spinal cord , somite , tail bud , trigeminal ganglion , [+]

  pax3 (paired box 3) gene expression in a Xenopus laevis embryo, NF stage 31/32, assayed via in situ hybridization, lateral view , anterior left, dorsal up.