|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
mef2c
|
|
laevis
|
NF stage 29 and 30
|
somite
,
myotome
|
dab2
|
|
laevis
|
NF stage 18
|
mesoderm
,
somite
|
dab2
|
|
laevis
|
NF stage 22
|
mesoderm
,
somite
|
dab2
|
|
laevis
|
NF stage 25
to
NF stage 29 and 30
|
notochord
,
somite
,
myotome
|
dab2
|
|
laevis
|
NF stage 35 and 36
|
heart
,
posterior cardinal vein
,
glomus
,
vascular plexus
|
dab2
|
|
laevis
|
NF stage 29 and 30
|
somite
,
myotome
|
myod1
|
|
laevis
|
NF stage 29 and 30
|
somite
,
myotome
|
myos
|
|
laevis
|
NF stage 29 and 30
|
somite
,
myotome
|
acta4
|
|
laevis
|
NF stage 29 and 30
|
somite
,
myotome
|
|
|
Fig. 8 Relationship of XDab2L and XDab2S and their spatiotemporal expression patterns. (a) Schematic representation of different domains of XDab2L and XDab2S. (b) Phylogenetic analysis of Dab1 and Dab2 proteins from the frogs, human, mouse, rat, cattle, and fish. (c) qPCR results showing XDab2L and XDab2S expression at different stages of the Xenopus laevis development with ornithine decarboxylase (odc) as the internal control. (d) Expression of XDab2L and XDab2L/XDab2S in Xenopus embryos at different developmental stages after whole-mount in situ hybridization. (e) Vibratome sections showing the expression of XDab2L/XDab2S (i, ii), XMyoD (iii, iv), XPax3 (v, vi), XMef2c (vii, viii), XMyos (ix, x), and XAC100 (xi, xii) after whole-mount in situ hybridization at stage 30. Boundaries of somites are indicated by arrows |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
mef2c
|
|
laevis
|
NF stage 31
to
NF stage 32
|
somite
,
pharyngeal arch
|
myf5
|
|
laevis
|
NF stage 31
to
NF stage 32
|
somite
,
pharyngeal arch
,
hyoid arch
,
chordoneural hinge
,
hypaxial muscle
,
[+]
|
actn4
|
|
laevis
|
NF stage 29 and 30
to
NF stage 31
|
presomitic mesoderm
,
somite
,
tail bud
|
myod1
|
|
laevis
|
NF stage 31
to
NF stage 32
|
presomitic mesoderm
,
somite
,
tail bud
|
myos
|
|
laevis
|
NF stage 31
to
NF stage 32
|
presomitic mesoderm
,
somite
,
peripheral retina
|
|
|
Fig. 9 Inhibition of XDab2 expression by morpholinos resulted in the downregulation of the expression of myogenic transcription factors in somites. Both blastomeres at the 2-cell stage were injected with control MO (a, c, e, g, i, k) or XDab2 MO (b, d, f, h, j, l), and the injected Xenopus embryos were fixed at stages 31–32 for the examination of the expression of XPax3 (a, b), XMyoD (c, d), XMef2c (e, f), XMyos (g, h), XAC100 (i, j), and XMyf5 (k, l) after whole-mount in situ hybridization |
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pax3 (paired box 3) gene expression in a Xenopus laevis embryo, NF stage 31/32, assayed via in situ hybridization, lateral view , anterior left, dorsal up. |