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Figure 6. Marker gene analyses of Insulin receptor substrate IRS-1-depleted embryos. A: IRS-1 knock down in neural tissue reduces the expression of the eye marker genes Rx1 and Pax6, but not the pan-neural marker gene Sox3 at stage 13. B: At stage 23, expression of Rx1, Pax6, and Otx2 in the eye is affected. In contrast, expression of Emx1, Krox20, and En2 in the brain is not affected. C: Coinjection of 0.5 ng of δ5′ untranslated region (UTR) IRS-1 RNA significantly reverted the IRS-1 morpholino oligonucleotide (MO) (20 ng) -induced reduction of Rx1 expression at stage 13. D,E: Quantitative representations are given. *P < 0.05, **P < 0.005, n, number of independent experiments; N, total number of embryos examined.Download figure to PowerPoint |
