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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
foxd4l1.1
|
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laevis
|
NF stage 11
|
dorsal marginal zone
|
zic3
|
|
laevis
|
NF stage 11
|
marginal zone
|
chrd
|
|
laevis
|
NF stage 10.5
|
dorsal marginal zone
|
zic2
|
|
laevis
|
NF stage 11
|
marginal zone
|
sox11
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural plate
,
sensorial layer
|
sox3
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neural plate
,
sensorial layer
|
otx2
|
|
laevis
|
NF stage 20
|
stomodeal-hypophyseal primordium
,
neuroectoderm
,
optic vesicle
,
neural plate
|
gsc
|
|
laevis
|
NF stage 10.5
|
dorsal marginal zone
|
egr2
|
|
laevis
|
NF stage 20
|
presumptive rhombomere
|
ncam1
|
|
laevis
|
NF stage 19
to
NF stage 20
|
optic vesicle
,
neural fold
,
presumptive rhombomere
|
gmnn
|
|
laevis
|
NF stage 11
|
marginal zone
|
vegt
|
|
laevis
|
NF stage 11.5
|
mesoderm
,
marginal zone
,
involuting marginal zone
|
nodal3.1
|
|
laevis
|
NF stage 10.5
|
dorsal marginal zone
|
|
|
Figure S5. Germ layer marker gene expression in xSuv4-20h double morphants. (A) Immuno-histochemistry on ctrl-MO and xSuv4-20h double-morphant tadpoles. Panels show representative cross-sections of neural tubes stained with antibodies against the histone epitopes indicated on top. Inj injected side. Squares on double-morphant sections represent the croped pictures shown in Figure 1D. (B-F) RNA in situ hybridization analysis of ctrl-MO injected and double morphant embryos at the indicated stages using probes against Chordin, Xnr-3 and Gooscoid (B), Krox20 and Otx2 (C), Pax-6 (D), N-CAM (E), FoxD5, Geminin, Zic2, Zic3, Sox3, Sox11 and VegT (F). Chordin, Xnr-3 and Gooscoid dorsal side views; animal pole is on the top. Krox20 - dorsal views, anterior on the left. Otx2 - anterior views, dorsal on the top. Pax-6 head region; rescued embryos included. N-CAM - dorsal views of stained embryos with the anterior on the left; rescued embryos included. FoxD5, Geminin, Zic2, Zic3 - dorsal views; injected halves are lineage-traced by coinjection of LacZ mRNA and subsequent βal staining (light blue). Sox2, Sox3 - dorsal views, anterior to the left. VegT - internal stain in bisected embryos. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tbxt
|
|
laevis
|
NF stage 11
|
mesoderm
,
marginal zone
|
dll1
|
|
laevis
|
NF stage 13
|
neuroectoderm
,
circumblastoporal collar
,
neural plate
|
a2m
|
|
laevis
|
NF stage 15
|
endoderm
,
central endoderm
|
sox17a
|
|
laevis
|
NF stage 15
|
endoderm
,
central endoderm
|
neurog2
|
|
laevis
|
NF stage 12.5
|
neuroectoderm
,
neural plate
,
neural plate border
|
tubb2b
|
|
laevis
|
NF stage 15
|
neuroectoderm
,
neural plate
,
Rohon-Beard neuron
,
neural plate border
,
interneuron
,
[+]
|
rax
|
|
laevis
|
NF stage 29 and 30
to
NF stage 33 and 34
|
retina
,
cement gland
,
pineal gland
|
myod1
|
|
laevis
|
NF stage 15
|
presomitic mesoderm
,
paraxial mesoderm
|
|
|
Figure S8. Xenopus laevis Suv4-20h1 or h2 mRNA overexpression. (A) Overexpression of frog Suv4-20h1 and h2 enzymes causes an upregulation of H4K20me2 and H4K20me3 marks. Bulk histones from uninjected embryos or embryos bilaterally injected with increasing amounts of Suv4-20h1 or h2 mRNAs were isolated at NF11.5 and analysed by Western blot. Pan H3 antibody was used as loading control. (B, C) Morphological phenotypes of NF30-33 embryos injected with xSuv4-20h1 (B) or h2 (C) mRNA. (D) RNA In situ hybridization of NF30-33 uninjected embryos (top row) and embryos injected with Suv4-20h1 (middle row) or h2 (bottom row) mRNA using probes against Rx-1 and Pax-6. Pictures show the head of stained embryos. (E) RNA In situ hybridization analysis of uninjected embryos (top row) and embryos injected with xSuv4-20h1 (middle row) or h2 (bottom row) mRNA using probes against Ngnr-1a, Delta-like 1, N-tubulin, Xbra, MyoD, Sox17 α and Endodermin. Pictures show dorsal views of stained embryos, anterior is on the left; Xbra pictures show vegetal views of NF11 embryos; MyoD pictures show dorsal views of NF15 embryos, with the head on the left. For Sox-17 α and Endodermin sagittal sections of NF15 embryos were created; pictures show internal view of the injected halves, with anterior on the left. |