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Figure 5. Xenopus TAF1β Is Expressed in the Embryonic Ectoderm and Is Required for Proper Ectodermal Differentiation(A–C) Stage 17 embryos stained by in situ hybridization for NFPC (A) or TAF1β (B and C) and viewed in cross-section. TAF1β is expressed in the neural plate (np), ventral ectoderm (ec), and presomitic mesoderm (psm). Within the ventral ectoderm, NFPC and TAF1β show similar expression patterns, with both highly expressed in the inner layer (arrows).(D) Stage 17 embryo immunostained with a TAF1 monoclonal antibody and viewed in whole-mount. TAF1 is expressed in both the nucleus and at the cell membrane.(E–H) Antisense NFPC and TAF1β morpholinos disrupt ectodermal differentiation. Embryos were coinjected with NFPCMO (E), TAF1βMO (F), or CMO (G), together with nLacZ RNA, fixed at stage 22, stained for β-galactosidase activity, and sectioned. Compared to CMO-injected embryos, embryos injected with NFPCMO or TAF1βMO exhibit similar ectodermal defects.(H) Immunoblot analysis of morpholino-injected embryos. Compared to uninjected (lane 1) or CMO-injected (lane 2) embryos, injection of TAF1βMO (lane 3) or NFPCMO (lane 4) significantly reduces the amount of TAF1β and NFPC protein present in the embryos, respectively.(I–L) TUNEL assay for apoptotic nuclei in the ectoderm. Embryos were injected as in (E)–(G), stained for β-galactosidase activity, and then subjected to whole-mount TUNEL assay, sectioned, and analyzed for apoptotic nuclei (dark blue nuclei, arrowheads). NFPCMO-injected (I) and TAF1βMO-injected (J) embryos exhibit fewer apoptotic nuclei in the ectoderm, as compared to CMO-injected embryos (K).(L) Results shown are the mean number of TUNEL-positive nuclei per section, averaged over 8–12 embryos per morpholino injection.The following abbreviations were used: nc, notochord; sm, somite. |
