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Fig. 3. NFPC is expressed in embryonic ectoderm. Embryos at (a,e) stage 14, (b,f) stage 16, (c,g) stage 18 and (d,h) stage 28 were stained by in situ hybridization for NFPC and viewed (a-d) in whole mount or (e-h) in cross-section. (a,e) At stage 14 (early neural plate), NFPC was restricted to the ventral ectoderm and was not expressed in the neural plate (np). In (e), the right-hand side of the np dorsal side of the embryo is shown. (b,f) As nc the neural plate folds to become the neural tube (stage 16), NFPC was expressed in the prospective epidermis and at the tips of the neural folds (arrow), and this pattern continued as the neural folds meet in the dorsal midline (c,g). (f,g) Transverse sections through stained embryos revealed that, within the ectoderm, NFPC was more highly expressed in the inner layer (arrowhead). (d,h) At tailbud stages, NFPC was expressed in the epidermis, branchial arches (ba), lens, otic vesicle (ov), and also within presumptive motor neurons (white arrow in h) in the caudal neural tube (nt) and in the dorsal fin. Other abbreviations: nc, notochord; psm, pre-somitic mesoderm. |
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Fig. 4. Comparison of NFPC expression with known ectodermal markers. Stage 18 embryos were analyzed by in situ hybridization for expression of (a,e) NFPC, (b) Xslug, (c) E-cadherin, or (d,f) epidermal keratin. NFPC, E-cadherin and epidermal keratin showed similar expression patterns in whole mount, expression being restricted to the ventral ectoderm and excluded from the neural plate and placodal region, as marked by expression of Xslug (b). NFPC was also uniquely expressed within the neural folds, however; arrow in (a). Transverse sections revealed that, within the ectoderm, NFPC was most highly expressed in the sensorial layer whereas epidermal keratin was most highly expressed in the outer layer; arrowheads in (e,f). |
