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Fig. 1. Pcdh7 is expressed in a subset of neural crest cells. Embryos at stage 17/18were subjected to in situ hybridization for Pcdh7 (A, F), or the neural crestmarker Snail2 (B, G) or double
in situ for Pcdh7 and Snail2 (C, D, H) or the neural crestmarker Sox9 (E, I). Embryos are viewed inwholemount (A–E) or in cross-section (F\\I). Pcdh7 is expressed in the sensorial layer of
the ectodermand at the tips of the neural folds (A, arrows in F), with highest expression along the lateral border of the neural crest. Snail2 is expressed in the neural crest, which derives
fromthe sensorial layer (B, G). Embryos subjected to double in situ for Pcdh7 and Snail 2 or Sox9 (C, D, E) reveal overlap along the lateral border of the neural crest (arrowheads),which is
confirmed in cross section (H, I). Anterior is the right in A–E. Sections F\\I are anterior, through the prospective hindbrain region. A summary diagram of the domains of the neural plate,
neural crest and Pcdh7 is shown (J). Abbreviations: np, neural plate; nc, notochord; psm, presomitic mesoderm. |
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Fig. 6.Wnt11b is disrupted in Pcdh7MO-injected embryos. Embryos were analyzed by in situ hybridization for Wnt11b (A) or double in situ hybridization for Pcdh7 and Wnt11b (B),
demonstrating that Wnt11b and Pcdh7 expression in the sensorial layer overlap at the neural crest border (arrows in B). To examine the effect of Pcdh7 knockdown on Wnt11b
expression, embryos were injected with Pcdh7MO, together with LacZ mRNA, fixed at stage 17/18, stained for β-galactosidase, and subjected to in situ hybridization for Wnt11b (C).
Results demonstrate that Pcdh7MO-injected embryos exhibit reducedWnt11b expression on the injected side (top of photo in C), as compared to CMO-injected embryos (D). |
