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Fig. 6. Expression of PCNA. The embryos in (A and C), (H), and (J and K) are oriented anterior to the left and dorsal to the top; (B) is a dorsal view, oriented anterior to the left; those in (E–G) are dorsal views oriented anterior toward the top. (A) Stage 20 embryo. (B) Stage 22 embryo. (C) Stage 26 embryo. (D) Stage 26 embryo, anterior view with dorsal located to the top. (E) Stage 28 embryo. Arrowheads demarcate stronger signal in the tailbud region. (F) Stage 28 embryo. (G) Stage 37 embryo. The bracket demarcates the right half of the CNS, and the left margin of the bracket aligns with the inner boundary of the ventricular zone. (H) Stage 37 embryo. The stain in the midbrain is located at the midbrain–hindbrain boundary. (I) Section through a stage 37 embryo at the level of the midbrain/hindbrain boundary, with the dorsum oriented to the left. This section was taken from the temporal margins of the optic cup, and therefore does not include any ciliary marginal zone stain. (J) Sense control stage 23 embryo. (K) Sense control stage 37 embryo. cg: cement gland, cns: central nervous system, m/h: midbrain/hindbrain boundary. All other labels follow that listed in the legend to Fig. 1. The scale bar in (G) equals 430 mm in (A and B), 550 mm in (C), 250 mm in (D), 690 mm in (E), 780 mm in (F), and 200 mm in G, 380 mm in (H),(J and K), and 100 mm in (I). |
