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Figure 4. 5hmC-expressing cells correspond to neurons but not to proliferative cells at larval (St50) and juvenile (St66) stages in Xenopus.
A–D1 and I–L1: 5hmC and PCNA double immunohistochemistry on the larval (A–D1; thalamic area) and juvenile (I–L1; dorsal telencephalic
area) brains showing that proliferative cells from the ventricular layer do not display the 5hmC mark at larval (A–D1) or juvenile stages
(I–L1). A1–D1 and I1–L1: High-power views of the respective framed boxes in A–D and I–L. Arrows point to 5hmC-negative proliferative
cells. E–H1 and M–P1: 5hmC and acetylated tubulin double immunohistochemistry on the larval (E–H1; thalamic area: th) and juvenile
(M–P1; dorsal telencephalic area) brains showing that 5hmC-expressing cells mainly correspond to neurons as shown at larval (E–H1) and
juvenile stages (M–P1). E1–H1 and M1–P1: High-power views of the respective framed boxes in E–H and M–P. Arrows point to ventricular
cells that do not exhibit acetylated tubulin labeling. lp, lateral pallium; ls, lateral septum; lv, lateral ventricle; mp, medial pallium, th: thalamus.
Scale bar590 lm for A–D,E–H,I–L,M–P; 35 lm for A1–D1,E1–H1,I1–L1,M1–P1. |
