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Fig. 7. katnal2is required for telencephalon development inX. tropicalis. A) Representative stage 46 X. tropicalis tadpole, following 1 pmol katnal2 morpholino injection into half of the embryo, stained for β-tubulin to visualize the nervous system. B) Close-up of the telencephalic region in A. C) Quantification of telencephalon size, comparing the uninjected side of the tadpole to the injected side, reveals reduced size on the morpholino-injected side (p < 0.0001; n = 37). D) Representative stage 46 X. tropicalis tadpole following 1 pmol katnal2 morpholino injection into half of the embryo, stained for proliferating cell nuclear antigen (PCNA) to visualize the proliferative cells of the nervous system, shows a reduction of area on the morpholino injected side. E) Close up of the telencephalic region (first ventricle) from D. F) Quantification of PCNA positive area in the telencephalic region (first ventricle), comparing the uninjected side to the injected side (p < 0.0001; n = 25). G) Transverse section of telencephalic region, stained for PCNA (ventricular zone progenitors, green) and vGLUT1 (glutamatergic neurons, magenta). |
