|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
pitx1.L
|
|
laevis
|
NF stage 12
to
NF stage 12.5
|
dorsal marginal zone
|
|
pitx1.L
|
|
laevis
|
NF stage 12
to
NF stage 13
|
ectoderm
,
cement gland primordium
,
anterior
,
non-neural ectoderm
|
|
pitx1.L
|
|
laevis
|
NF stage 16
|
ectoderm
,
cement gland primordium
,
neural groove
,
anterior
|
|
pitx1.L
|
|
laevis
|
NF stage 25
|
cement gland
,
eye primordium
|
|
pitx1.L
|
|
laevis
|
NF stage 28
|
stomodeal-hypophyseal primordium
,
cement gland
,
optic vesicle
|
|
pitx1.L
|
|
laevis
|
NF stage 33 and 34
|
stomodeal-hypophyseal primordium
,
cement gland
,
olfactory region
|
|
| |
FIG. 3. Expression of xPitx1 detected by whole-mount in situ hybridization. All gastrula to early neurula stage embryos are positioned with the yolk plug to the rear with the presumptive anterior regions facing up (with the exception of B, anterior indicated and dorsal up). (A, B) At gastrulation, transcript is detectable above the dorsal lip (dark arrows). (C) Expression is detected in the presumptive anterior ectoderm as a band. An earlier forming dot of expression sits in the central region of the presumptive head plate out of view on the top of this stage-12 specimen. (D) Late gastrula/early 12.5 neurula expression of xPitx1 is detected as a dot in the center of the neural plate as the anterior band forms a crescent shape. (E) High expression of xPitx1 occurs in the cement gland anlage at stage 16. (F, G) Expression of xPitx1 continues within the cement gland (cg) and stomodeum (st) as transient expression commences in the optic cup (e, arrow in E) and olfactory epithelia during organogenesis through to tailbud stages.
Reproduced with permission of the Publisher, John Wiley & Sons |
|
|
| |
pitx1 (paired-like homeodomain 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 16, anterior view, dorsal up.
Reproduced with permission of the Publisher, John Wiley & Sons. |
|
|
| |
pitx1 (paired-like homeodomain 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 23, lateral view, anterior right, dorsal up.
Reproduced with permission of the Publisher, John Wiley & Sons. |
|
|
| |
pitx1 (paired-like homeodomain 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior right, dorsal up, head and trunk region only.
Reproduced with permission of the Publisher, John Wiley & Sons. |
|
|
| |
pitx1 (paired-like homeodomain 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33& 34, lateral view, anterior right, dorsal up, head and trunk region only.
Reproduced with permission of the Publisher, John Wiley & Sons. |
