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Fig. 5. PGC-directed sox7 knockdown or overexpression altered germ plasm localization in early PGC development. Control, sox7-MO, sox7-FL, and
sox7-MO rescue embryos were analyzed for Piwi (germ plasm; pink) and PCNA (nuclear; green) expression by immunofluorescence at the indicated stages.
(A) Germ plasm localization in each PGC was classified as membrane, nuclear or dispersed and quantified; n numbers of PGCs analyzed are indicated.
(B) Representative images of each classification are shown for all stages analyzed. Representative PGC in each panel is outlined in white. *P<0.05, **P<0.005
(compared with ctrl); #
P<0.05 (compared with sox7-MO). ctrl, control; FL, full length, flag-tagged; MO, morpholino. All images were taken at 20×.
Scale bars: 100 μm. |
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Fig. 6. PGC-directed sox7 knockdown or
overexpression increased the number of
transcriptionally active PGCs in early
development. Control, sox7-MO, sox7-FL,
and sox7-MO rescue embryos were analyzed
for Piwi (germ plasm; pink) and pSer2
(transcriptionally active nuclei; green)
expression by immunofluorescence at the
indicated stages. (A) The percentage of
pSer2-positive PGCs was determined;
n numbers of PGCs analyzed are indicated.
(B) Representative images of PGCs from
control and injected embryos are shown for
each stage. Representative PGC in each panel
is outlined in white; arrowheads indicate pSer2-
positive PGCs; arrows indicate pSer2-positive
somatic cell nuclei. *P<0.05, **P<0.005
(compared with ctrl); #
P<0.005 (compared with
sox7-MO). ctrl, control; MO, morpholino; FL, full
length, flag-tagged; pSer2, RNA polymerase II
carboxy-terminal-domain phosphorylated
at Ser2. All images were taken at 20×. Scale
bars: 50 μm. |
