|
Gene |
Clone |
Species |
Stages |
Anatomy |
pkd2.L
|
|
laevis
|
NF stage 1
|
animal pole
|
pkd2.L
|
|
laevis
|
NF stage 3 (4-cell)
|
animal pole
|
pkd2.L
|
|
laevis
|
NF stage 10
|
mesoderm
,
deep
|
pkd2.L
|
|
laevis
|
NF stage 11.5
|
presumptive axial mesoderm
|
pkd2.L
|
|
laevis
|
NF stage 12.5
|
mesoderm
,
upper blastopore lip
,
axial mesoderm
|
pkd2.L
|
|
laevis
|
NF stage 18
|
notochord
,
circumblastoporal collar
,
gastrocoel roof plate
,
neural plate
,
sensorial layer of neurectoderm
,
[+]
|
pkd2.L
|
|
laevis
|
NF stage 18
|
notochord
,
circumblastoporal collar
,
gastrocoel roof plate
,
neural plate
,
sensorial layer of neurectoderm
,
[+]
|
|
|
Figure 1. Expression of pkd2 mRNA during Xenopus Embryogenesis.
(A–C) Maternally deposited mRNA localized to the animal hemisphere of the zygote (A, A′) and four-cell stage embryos (B). Note animal-vegetal shift of expression as indicated in the zygote. (C) Lack of a specific signal in specimen hybridized with a sense probe.
(D–F) pkd2 transcripts in deep mesodermal layers of the early gastrula (D, D′, D′; black arrowheads; plane of section indicated in D by dotted line), in dorsal notochordal mesoderm at mid gastrula (E), and in tail organizer (white arrow) and posterior notochord at late gastrula stages (F, F′). Please note the lack of transcripts in the dorsal lip and the SM (white arrowheads). Border of inner and outer layer of marginal zone indicated by dotted line. Yolk plug and archenteron roof indicated by dashed lines.
(G) Deep neuroectodermal, tail organizer, and posterior notochordal expression at neurula stages; no expression of GRP detected (G4).
a, anterior; an, animal; ar, archenteron; bf, blastocoel floor; cbc, circumblastoporal collar; dl, dorsal lip; e, endoderm; el, epithelial layer (of cbc); l, left; n, notochord; ne, neuroectoderm; psm, presomitic mesoderm; r, right; s, somites; v, ventral; veg, vegetal; yp, yolk plug. |
|
Gene |
Clone |
Species |
Stages |
Anatomy |
pkd2.L
|
|
laevis
|
NF stage 19
|
neuroectoderm
,
anterior neural tube
|
pkd2.L
|
|
laevis
|
NF stage 20
|
intermediate mesoderm
,
anterior neural tube
|
pkd2.L
|
|
laevis
|
NF stage 23
|
spinal cord
,
intermediate mesoderm
,
floor plate
,
anterior neural tube
|
pkd2.L
|
|
laevis
|
NF stage 33 and 34
|
tail tip
|
pkd2.L
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
pronephric nephrostome
,
pronephric duct
,
pharyngeal arch
,
pronephric kidney
,
[+]
|
|
|
Figure S1: Related to Figure 1. Late expression pattern of pkd2.
Expression in the neuroectoderm (A-C), intermediate mesoderm (B, C), pronephric system (D), branchial arches (D’) and tailbud (E) in early to late tailbud stages.
Key: a, anterior; ba, branchial arches; d, dorsal; e, endoderm; fp, floorplate; im, intermediate mesoderm; l,left; lpm, lateral plate mesoderm; mhb, mid hindbrain boundary; n, notochord; ne, neuroectoderm; pd,
pronephric duct; pn, pronephros; r, right; s, somites; v, ventral |
|
|
|
pkd2 (polycystin 2, transient receptor potential cation channel) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 19, lateral view, anterior left, dorsal up.
Key: ne= neuroectoderm. |
|
|
|
pkd2 (polycystin 2, transient receptor potential cation channel) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 20, lateral view, anterior left, dorsal up.
Key: im, intermediate mesoderm. |
|
|
|
pkd2 (polycystin 2, transient receptor potential cation channel) gene expression in bisected Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up.
Key: fp, floorplate; im, intermediate mesoderm; l,
left; lpm, lateral plate mesoderm; n, notochord; ne, neuroectoderm; pd,
pronephric duct; pn, pronephros. |
|
|
|
pkd2 (polycystin 2, transient receptor potential cation channel) gene expression in Xenopus laevis embryo, head region, assayed via in situ hybridization, NF stage 36, and tail tip at NF stage 34, lateral view, anterior left, dorsal up.
Key: pn, pronephric nephrostomes; ba, pharyngeal/branchial arches; mhb, mid hindbrain boundary. |
|
|
|
pkd2 (polycystin 2, transient receptor potential cation channel) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 19, lateral view, anterior left, dorsal up.
Key: pd, pronephric duct. |