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Fig. 5. Pax3 or Zic1 morpholino mediated knockdown blocked Pax3-Zic1 targets expression at the neurula stage: (A) Pax3MO (40 ng) or Zic1MO (40 ng) were injected in one blastomere at the 2-cell stage embryos. Embryos were collected at the neurula stage (stage 15–18) and analyzed by ISH (upper left). (B) ISH with a sox8 probe was used as a positive control for the activity of each morpholino. (C) The 10 Pax3-Zic1 targets analyzed showed reduced expression upon Pax3 or Zic1 knockdown. All panels are showing dorsal views, anterior to top. The injected side is on the right as indicted by the presence of the lineage tracer (red staining). |
