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Figure 2. Pnhd transcript localization at different developmental stages. WISH was carried out with albino embryos using pnhd antisense and sense RNA probes. (A) Vegetal view of a stage 10 embryo (dorsal is up). Arrowhead points to vegetal endoderm. (B) Cross-section of a stage 10 embryo. (C) Control embryo, stage 10 (sense probe). (D) Vegetal view of a stage 11.5 embryo (dorsal is up). (E) Vegetal view of a stage 12 embryo (dorsal side is up). Arrows in A,D,E indicate mesodermal expression. (F) Dorsal view of a stage 14 embryo (anterior is up). Arrowhead marks the neuroectoderm. (G) Cross-section of the embryo shown in F. (H) Anterior view of a stage 19 embryo (dorsal is up). (I) Posterior view of a stage 19 embryo (dorsal is up). Arrow indicates staining in the tailbud. (J) Side view of a stage 25 embryo. (K) Head of a stage 25 embryo (anterior is left). Arrowhead points to the signal in the superficial ectoderm cells that are dorsal to the cement gland. (L) Cross-section corresponding to the midbrain level of embryo in J. (M) Dorsal view of a stage 25 embryo. (N) Stage 25, control sense probe. (O) Lateral view of a stage 25 embryo tailbud. Anterior is to the left in J,K,M-O. (P) Transverse section of a stage 25 embryo corresponding to J and O. Dashed lines mark the approximate level of corresponding sections (indicated by bold letters). Dorsoventral (D/V) and anteroposterior (A/P) embryonic axes are indicated. Abbreviations: bc, blastocoel; bv, brain ventricle; cg, cement gland; df, dorsal fin; dl, dorsal blastopore lip; en, endoderm; ev, eye vesicle; lm, lateral mesoderm; me, mesoderm; mhb, midbrain-hindbrain boundary; ne, neuroectoderm; nt, neural tube; ntb, neural tube border; no, notochord; ov, otic vesicle; s, somites; sm, somitic mesoderm; tb, tailbud. Scale bars: 50 µm (B,G,L); 25 µm (P). |
