| |
Fig. 3. Stage 29/30 and 31/32: High-magnification
photomicrographs of 20,um sections showing POMC
mRNA in pituitary primordium (short arrows) and
posterior diencephalon (pDI, long arrows) using exonic
probe 2 (A,B), but no primary transcript with intronic
probes 4 or 5 (C,D). (A) Posterior coronal section from a
stage 29/30 embryo (the sixth of eight with labeling in
pituitary plate) showing two midline foci of POMC mRNA
in brain adjacent to labeled plate. (B) Similar section at
stage 31/32 showing higher levels of POMC mRNA in
plate and diencephalon. (C,D) Intronic probes 4 and 5
showed no above background labeling of plate (arrows) or
brain at stage 29/30 and 31/32, respectively. Note, these
thicker sections always show an increased background grain
labeling, but also an enhanced signal to noise. Calibration
bar is 40um. |
