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Fig. 5. In situ hybridization of sagittal sections of the pituitary (arrowhead) of stage-62 brains. (A) Hematoxylin and eosin stain. (B–J) In situ hybridization. (B)
D2 in situ in a section adjacent to A. (C) Higher magnification of D2 in the pituitary in B. (D) TSHb. (E) POMC. (F) Simultaneous in situ with POMC (orange) and
TSHb (purple). (G–J) Stage-58 tadpoles treated for 4 days with methimazole and iopanoic acid. (G and H) Simultaneous POMC and TSHb in situ, but both probes
were labeled with digoxigenin. (G) Stage-58 tadpoles treated for another 4 days with 10 nM T4. (H) Same as G, but treated for another 4 days with 10 nM T3.
(I) D2 in situ of doubly inhibited stage-58 tadpole. (J) Same as I except treated for 4 days with 10 nM T4. (Bar for A and B 5 200 mm.) (Bar for C–J 5 100 mm.) In
all figures, anterior is left and dorsal is up. |
