| |
Fig. 3. In situ hybridization using
a Xenopus POMC cDNA probe.
Stage 46 larvae were examined as
described in Methods: experiments
were performed with the
POMC (A and C; C is a higher
magnification of A) or pBR 328
probe (B). Immunodetection of
digoxigenin-labeled DNA reveals
only POMC transcripts. These
are highly localized in a region
representing the future pars intermedia
of the pituitary. This is in
line with the results of the dissection
experiment described above
(see Fig. 2B), where the POMC
transcripts were shown to be expressed
only in the head. Several
anatomical structures are indicated
in B |
