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Gene/CloneSpeciesStageAnatomy ItemExperimenter
prkcixenopus foregut [+] 

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Experiment details for prkci

Li Y et al. (2008) Assay

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Gene Clone Species Stages Anatomy
prkci.L laevis NF stage 13 to NF stage 17 foregut

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  Figure 5. Sfrp5 is required to establish a foregut epithelium. (A,B) Removal of the neural plate at stage 13 reveals the archenteron floor foregut endoderm in control embryos (A) is a flat epithelial sheet of cells, while in sfrp5-MO embryos (B) the cells round up and are loosely adherent. Embryos are oriented anterior down. (C–H) Confocal immunostaining of anti-β-catenin (C,F), anti-atypical-PKC (D,G), and anti-C-cadherin (E,H) in the foregut region of midsagittally bisected control (C–E) and sfrp5-MO-injected (F–H) embryos at stage 20 (anterior left, dorsal up). Nuclei are counterstained in green. Insets show a high magnification of the foregut surface cells. (I–T) Confocal immunostaining of aPKC (I–N) and β-catenin (O–T) in the foregut and hindgut surface of control and Sfrp5-depleted embryos (anterior left) at the indicated stages. (U–W) The schematic shows the regions of the foregut (blue box) and hindgut (yellow box) imaged in I–T. The red line indicates the regions of the foregut and hindgut surface that exhibit a polarized epithelium with basolateral β-catenin and apical aPKC. The loss of Sfrp5 results in a failure of the foregut epithelium, reduced cell adhesion and a loss of apical–basal polarity. (fgc) Foregut cavity; (ar) archenteron; (bc) blastocoel.