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Fig. 7. Grhl3 can expand the expression of superficial markers and inhibit the expression of deep cell markers. Embryos were injected with GFP (A�D and
I�L) or Grhl3 (E�H and M�P) and LacZ as a lineage marker. Embryos were fixed at neural plate stages, stained for B galactosidase (green) and for the
in situ marker (purple), as indicated. The sections are transverse and show the epidermal ectoderm except for N tubulin which shows the neural ectoderm.
Grhl3 but not GFP control injections caused an expansion of the superficial cell markers (E�H). An exception was XIRG which unlike the other
superficial cell markers showed a very weak response to Grhl3 which was limited to a few cells (arrows). Grhl3 also caused an inhibition of the deep cell
markers (M�P). The cells with strong reduction in expression clustered together producing apparent holes/bubbles in the epidermis (M+N, arrows). Grhl3
caused a thickening of the epidermis, the boundaries of the epidermis are highlighted with black bars. |
