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Fig. 1. Lipid Rafts in the Pronephric Kidney. (A,B) Whole mount immunostaining of Xenopus pronephroi using the B-subunit of Cholera Toxin (CT-B) or an antibody against Caveolin-1 at stage 42. Proximal tubules were counterstained using the 3G8 antibody (A) or ECL (B). pt, proximal tubules; dt, distal tubules. (C–H׳) Cryostat sections of stage 35, 39 and 42 Xenopus embryos stained with CT-B and either 3G8 (C–F׳) or 4A6 (G–H׳). (I–N׳) Paraplast sections of stage 35, 39 and 42 Xenopus embryos stained with Caveolin-1 and 3G8 (I–L׳) or 4A6 antibodies (M–N׳). The gray-scale panels show CT–B and Caveolin-1 staining only, while the color panels show the merged images. (O–S׳) Paraplast sections of stage 40 Xenopus embryos stained with anti-Caveolin-1 in combination with ECL (O–P׳), Clathrin (Q,Q׳), Rab5 (R,R׳) and Rab7 (S,S׳). In panel P and P׳ co-localization of Caveolin-1 and ECL staining in the apical domain was analyzed using the co-localization module of the LAS AF software and is indicated by white pixels. The panels F,F׳,H,H׳,L,L׳,N,N׳,O׳,P׳,Q׳,R׳ and S׳ are close-ups of the areas indicated by the white boxes. |
