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Figure 4. Alteration of the expression of genes controlling migration/adhesion in Rx1 gain- and loss-of function experiments. A–I: Embryos injected unilaterally with control standard morpholino (CoMo; A, D, G), MoRx1 (B, E, H), or Rx1 (C, F, I) and analyzed at early neurula stage. Frontal views, dorsal to the top. The probes used are indicated in the top right corner of each panel. J: Relative quantification by RT-qPCR of Sh2d3c, Efnb1, and Fat1 in embryos injected with GFP alone (GFP), or coinjected with either control standard morpholino and GFP (CoMo) or MoRx1 and GFP (MoRx1). Error bars indicate SEM. *P < 0.05; **P < 0.01 (Student's t-test). No significant difference is observed between GFP and CoMo samples.Download figure to PowerPoint |
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sh2d3c (SH2 domain containing 3C) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 19, anterior view, dorsal up. |
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sh2d3c (SH2 domain containing 3C) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33, lateral view, anterior left, dorsal up. |