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sh2d3cxenopus   

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Experiment details for sh2d3c

Giudetti G et al. (2014) Assay

Characterization of the Rx1-dependent transcriptome during early retinal development.

Gene Clone Species Stages Anatomy
sh2d3c.L laevis NF stage 15 neural groove , neural plate

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  Figure 4. Alteration of the expression of genes controlling migration/adhesion in Rx1 gain- and loss-of function experiments. A–I: Embryos injected unilaterally with control standard morpholino (CoMo; A, D, G), MoRx1 (B, E, H), or Rx1 (C, F, I) and analyzed at early neurula stage. Frontal views, dorsal to the top. The probes used are indicated in the top right corner of each panel. J: Relative quantification by RT-qPCR of Sh2d3c, Efnb1, and Fat1 in embryos injected with GFP alone (GFP), or coinjected with either control standard morpholino and GFP (CoMo) or MoRx1 and GFP (MoRx1). Error bars indicate SEM. *P < 0.05; **P < 0.01 (Student's t-test). No significant difference is observed between GFP and CoMo samples.Download figure to PowerPoint

Gene Clone Species Stages Anatomy
sh2d3c.L laevis NF stage 19 neural plate , optic field

  sh2d3c (SH2 domain containing 3C) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 19, anterior view, dorsal up.

Gene Clone Species Stages Anatomy
sh2d3c.L laevis NF stage 33 and 34 retina , brain

  sh2d3c (SH2 domain containing 3C) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33, lateral view, anterior left, dorsal up.