| |
Figure 1. Xshh expression in the developing brain. A–C: Lateral views of Xshh mRNA expression (blue) in stage 32, 34, and 38 embryonic brains, respectively. D:Xshh is found at the level of the midbrain in a transverse section of a stage 38 Xenopus embryo. Dotted red line denotes the presumptive location of the optic tract. E: A ventral view of HRP-labeled retinal axons (brown), along with Xshh expression (blue) in a stage 38 brain, at the optic chiasm. F: A lateral view of the same co-labeled brain, along the optic tract, with the white arrow at RGC axons, midway across the diencephalon. Unless otherwise noted, in all appropriate figures, dorsal is to the top. fp, floor plate; no, notochord; nt, neural tube; oc, optic chiasm; ot, optic tectum. Scale bar in D = 100 μm, and in F = 40 μm. |
