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Fig. 9. Shroom family proteins and cell elongation in non-neural cells. (A) In situ hybridization reveals Shroom1 expression in the cement gland. (B) In situ hybridization reveals Shroom3 expression in the cement gland, and also the invaginating nasal and otic placodes. (C) Cross-section through the cement gland, stained with phalloidin to reveal cell shapes. (c′) A schematic diagram of the section in C, illustrating the cement gland epithelial cells (red) and other surrounding tissues (grey). (D) Graph of changes in apical constriction (blue) and apicobasal cell elongation (red) of cement gland cells over time (stages 19-25). (E)A schematic diagram illustrating the change in cement gland epithelial cell shape. (F) Projection of optical sections ofα -tubulin staining of cement gland epithelial cells. (f′)α -tubulin staining shown in F merged with propidium iodide staining to show nuclei. (G) Robust apical MT arrays in the cement gland are associated with a broad apical accumulation of γ-tubulin. (g′) γ-tubulin staining shown in G merged with propidium iodide staining to show nuclei. |
