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Figure 6. The alternative spliced isoforms of Xdhcr7 differentially affect eye development upon overexpression. a-d rdquo , k: Microinjection of Xdhcr7-M (2 ng) into one blastomere of two-cell-stage embryos. e-h rdquo , i-j rdquo , l-l rdquo : Microinjection of Xdhcr7-S (750 pg) into one blastomere of two-cell-stage embryos. In combination, 200 pg of beta -galactosidase mRNA was injected as linage tracer (blue staining). a, c, e, g: Anterior view (dorsal up); dashes indicate the midline. b, b rdquo , d, d rdquo , f, f rdquo , h, h rdquo : Lateral view (dorsal up). b', d', f', h': Frontal view (dorsal up). i, j, k, l: Dorsal view (anterior down). i', j', l'-l rdquo : Transversal sections. a-j': WMISH analysis of Xrx1/Xen2/Xkrox20 (a-b rdquo , e-f rdquo ), Xsix3 (c-d rdquo , g-h rdquo ), Xgsh1 (i-i') and Xnkx2.2 (j-j') expression. k-l rdquo : Eye phenotype. a-b rdquo , c-d rdquo : The expression of Xrx1/Xkrox20/Xen2 as well as Xsix3 is not affected upon overexpression of Xdhcr7-M. e: Xdhcr7-S does not affect the early expression of Xrx1, Xkro20 (r3 and r5), and Xen2 (mhb), although the late expression of Xrx1 is reduced (f-f rdquo , 30%, n =3 0). g: Slight expansion of the early expression of Xsix3 upon overexpression of Xdhcr7-S; however, the late expression (h-h rdquo ) is severely reduced (62%, n = 26). i-j': Xdhcr7-S causes a reduction of the expression of Xgsh1 (i-i'), but not obviously Xnkx2.2 (j-j'). k: No obvious alteration of eye formation is induced by Xdhcr7-M overexpression. l: Small eye phenotype induced by Xdhcr7-S (38%, n = 200). l'-l rdquo : The retinal patterning of the Xdhcr7-S-injected eye is not severely affected. cs, control side; is, injected side; le, lens; mhb, midbrain-hindbrain boundary; r3, rhombomere 3; r5, rhombomere 5; rpe, retinal pigmented epithelium. |
