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Fig. 2. (A–C) Wholemount in situ hybridization of xSix3 during development, showing the progression of the expression pattern from round patch of cells to a cross-shaped domain in the ventral-rostral forebrain. (D–L) Vibratome sections through the embryos shown in A–C. (D–F) By st14, xSix3 mRNA can be detected in a continuous domain in the sensorial layer of the neuroectoderm. (G–I) At st25, a sagittally-oriented prolongation that extends into the telencephalon (white arrowheads. (J–L) During the early cell differentiation stages of the nervous system, isolated group of cells in the forebrain (black arrowhead in J), and parts of the diencephalic mantle layer (black arrowhead in L) express xSix3. The white arrowhead in J shows the telencephalic prolongation. |
