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Fig 3. XSMOC-1 binds to pro-EGF and co-localizes with pro-EGF in vivo.(A) Immunoblot of pro-EGF following co-immunoprecipitation of pro-EGF with XSMOC-1, XSMOC-1 δEC, or XSMOC-1 EC in the presence of TBST/0.1% SDS; pro-EGF binds to XSMOC-1 and XSMOC-1 δEC, but not XSMOC-1EC. (B) RT-PCR analysis of HEK293 cells showing positive signal for pro-EGF(C) Representative confocal image showing co-localization of XSMOC-1 and pro-EGF (red fluorophore) on HEK293 cells using the PLA method. Nuclei are stained blue with DAPI. (D, E) Representative whole mount hybridization in situ images of Xenopus neurula embryos (stage 26) stained for XSMOC-1 (C) or pro-EGF (D). The locations of the eye (e) and pronephros (pn) are indicated. |
