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Figure 2. fus morphants form all germ layers, but have mesodermal differentiation defects. All pictures show lateral view with animal region up, except for A and E, which show an animal view, and C and G, which show a dorso-vegetal view. (A–H) Normal zygotic and localized gene expression in fus morphants. (A,E) Zygotic ectodermal tfap2 expression is normal in fus morphants. (B,F) Zygotic endodermal sox17b expression is normal in fus morphants. (C,G) Normal dorsal mesoderm expression of chd in fus morphants. Notice the superficial staining in fus morphants caused by lack of invagination of the mesoderm. (D,H) Normal marginal zone fgf8 expression in fus morphants. (I–N) Mesodermal differentiation defects in fus morphants. (I,J) bra is expressed circumferentially in the marginal zone in normal embryos but is essentially absent in fus morphants. (L,M) eomes expression is restricted to a narrow region of the dorsal mesoderm in normal embryos but is significantly elevated in fus morphants. (K) Rescue of bra expression in fus morphants by injection of MO-resistant fus mRNA. (N) Partial rescue of blastopore formation and eomes repression upon fus mRNA injection. |
