|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
slc5a8
|
|
tropicalis
|
NF stage 10.5
|
involuting marginal zone epithelial layer
,
upper blastopore lip
|
tfap2a
|
|
tropicalis
|
NF stage 10.5
|
ectoderm
,
animal cap
|
osr1
|
|
tropicalis
|
NF stage 10.5
|
central endoderm
,
ventral mesoderm
,
dorsal endomesoderm
|
sox17a
|
|
tropicalis
|
NF stage 10.5
|
endoderm
,
central endoderm
,
ventral endoderm
|
hnf1b
|
|
tropicalis
|
NF stage 10.5
|
endoderm
,
central endoderm
|
gdf3
|
|
tropicalis
|
NF stage 10.5
|
central endoderm
,
dorsal endoderm
|
nodal3.1
|
|
tropicalis
|
NF stage 10.5
|
involuting marginal zone epithelial layer
,
upper blastopore lip
,
dorsal endoderm
|
|
|
Figure 1. Sox17-regulated endoderm transcriptome.
(A) Endoderm expression of sox17a and sox17b in NF10.5 Xenopus tropicalis gastrula. (B–C) Sox17 immunostaining in control-MO (B) and sox17a/b-MO (C) injected gastrula with an antibody that recognizes both Sox17a and Sox17b shows MO effective knockdown. (D) Time course heatmap of Sox17-regulated transcripts. Differentially expressed genes from a pairwise comparison of control-MO and sox17-MO (>2 fold change, FDR < 5%) at each stage showing transcripts that are downregulated or upregulated early (NF9-10) or late (NF10.5–12). Key genes are color coded based on regional expression from fate map in (E). (E) Enriched expression of Sox17-regulated transcripts. Gastrula fate map (dorsal right) colored by different tissues. Stacked bar graphs showing different patterns of enriched spatial expression based on RNA-seq of dissected gastrula tissues (Blitz et al., 2017). Distribution of expression patterns statistically different from all genes in the genome was determined by two-sided Kolmogorov-Smirnov tests *p<0.01. (F) In situ validation of Sox17-regulated transcripts. Disrupted expression in sox17-MOs is rescued by co-injection of mouse (m) Sox17 RNA. |