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sox17b.1xenopus   

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Experiment details for sox17b.1

Wills A et al. (2008) Assay

Bmp signaling is necessary and sufficient for ventrolateral endoderm specification in Xenopus.

Gene Clone Species Stages Anatomy
sox17b.1 xenopus NF stage 10.5 endoderm , vegetal yolk mass

  Figure 3. Bmp antagonists disrupt early endoderm formation. One-cell embryos were injected vegetally with mRNA encoding the following Bmp Antagonists. B: DN-Alk3 (0.5 ng). C: Chordin (0.5 ng). D: Noggin (0.5 ng). The embryos were assayed by in situ hybridization with a probe for Sox17 beta at stage 10.5. A: A control injected with water. F-J: Two-cell-stage embryos were injected vegetally with 200 pg of lacZ mRNA, and mRNA for Chordin or Noggin as indicated. Embryos were fixed at stage 11, stained for lacZ using red gal substrate, and analyzed for Sox17 beta expression.