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Figure 2. Downregulation of transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-C) The expression of sox2 was not changed in all three conditions. (D-O) The expression of nscl-1 (E,F), neurod (H,I), aml1 (K,L), and emx1 (N,O) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (D,G,J,M). Panels (A-L) show dorsal views of neurula stage embryos (stage 15/16), and (M-O) are anterior views of tail bud stage embryos (stages 25 to 28). |
