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Display additional annotations [+]
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Figure 2. Hh/Gli Signaling Is Required for Respiratory Wnt-Mediated Specification in Xenopus
(A) Schematic of a section through the lung field showing a model of Hh/Gli regulation of Wnt/BMP-mediated respiratory induction.
(B) In situ hybridization and immunostaining of foregut sections from NF34 stage control and Hh/Gli-deficient Xenopus embryos generated by either Gli2+Gli3-MO injection or cyclopamine treatment from NF20–34. Expression of the lung-inducing wnt2/2b and bmp4 ligands and Nkx2-1+ respiratory specification requires Hh/Gli signaling, which can be rescued by injection of a human Gli2 mRNA. Foxf1+ lpm and nuclear pSmad1/5/8 are also reduced in Hh-deficient embryos. The scale bar represents 50 μM.
(C) Expression of ptch1 and wnt2b are activated in mesoderm explants treated with the Hh-pathway agonist SAG from NF20–34.
(D) Activation of β-catenin by Bio treatment from NF28–34 rescued and expanded (bracket) the nkx2-1 respiratory domain in Gli2+3 MO-injected embryos, indicating that Hh signaling acts upstream of Wnt. The red arrow indicated absent nkx2-1 expression. See also Figure S2. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
foxf1
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
head region
,
pronephric duct
,
foregut
,
lateral plate mesoderm
,
[+]
|
foxa2
|
|
laevis
|
NF stage 35 and 36
|
head region
,
mesoderm
,
endoderm
,
foregut
,
lateral plate mesoderm
,
[+]
|
bmp2
|
|
laevis
|
NF stage 35 and 36
|
brain
,
head region
,
mesoderm
,
foregut
,
pharyngeal region
|
fgf10
|
|
laevis
|
NF stage 35 and 36
|
head region
,
mesoderm
,
foregut
,
lateral plate mesoderm
,
neural tube
,
[+]
|
gata6
|
|
laevis
|
NF stage 35 and 36
|
mesoderm
,
endoderm
,
foregut
,
lateral plate mesoderm
|
gata6
|
|
laevis
|
NF stage 35 and 36
|
brain
,
mesoderm
,
endoderm
,
eye
,
foregut
,
[+]
|
bmp7.1
|
|
laevis
|
NF stage 35 and 36
|
brain
,
head region
,
mesoderm
,
pronephric duct
,
foregut
,
[+]
|
gata4
|
|
laevis
|
NF stage 35 and 36
|
endoderm
,
foregut
,
lateral plate mesoderm
|
fgf7
|
|
laevis
|
NF stage 35 and 36
|
head region
,
mesoderm
,
foregut
,
lateral plate mesoderm
,
neural tube
,
[+]
|
ptch1
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
head region
,
foregut
,
lateral plate mesoderm
,
neural tube
,
[+]
|
rdh10
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
head region
,
mesoderm
,
pronephric duct
,
eye
,
[+]
|
aldh1a2
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
pronephric duct
,
eye
,
lateral plate mesoderm
,
[+]
|
|
|
Figure S1 relevant to Figure 1. Additional analysis of gene expression in Xenopus foregut explants.
(A) Schematic of the Xenopus specification assay culturing foregut endoderm (yellow) and mesoderm (orange) together or separately from NF20 to NF36.
(B) In situ hybridization of stage NF36 embryos and explants using the indicated probes. The number of explants robustly expression the gene is indicated in the lower right of each panel. |