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Figure 1. Polarized and Nonpolar Neural Progenitors Have Significantly Different Cell-Cycle Kinetics Parameters(A) Dual pulse S phase labeling (DPSL) technique applied on wild-type NF13 Xenopus embryos (a) and analyzed by sectioning (b) shows that outer polar progenitors have shorter cell cycle (TC) and S phase (TS) lengths than inner nonpolar progenitors (c, mean ± SEM).(B) Similar embryos were processed for percentage of labeled mitoses (PLM, to estimate TG2+1/2M) (a–c). Representative sections are shown in (b), in which white arrowheads show labeled mitoses for nonpolar progenitors, while yellow arrowheads show labeled mitoses for polarized progenitors. During the time course of the experiment, neural plate cells converge to the midline. Percentage of mitotic indices for polarized and nonpolar progenitors showed no differences (d, mean ± SEM).(C) Table summarizes different cell-cycle parameters for the two types of progenitors. See the Experimental Procedures and Supplemental Experimental Procedures for details about the techniques and calculations of kinetic parameters. All scale bars represent 100 μM. |
