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Fig. 7. Knockout of zygotic Mov10 shows abnormal staining of neuronal
precursors in the ventricular zone. A: A stage 36 tadpole showing
the plane of sectioning for B through P. B: Representative fluorescence
image of control embryo shows the forebrain region with Sox3 positive
precursors (in red) surrounding the lumen of the ventricle. Note the
expanded dorsal Sox3 labeling in the ventricular zone, denoted by
white arrowheads. C: DAPI staining (in blue) of the same section shown
in B. D: Merged images from B and C. E–G: Representative fluorescence
images from a similar region, as shown in B, from representative
z-MO injected tadpoles. E: Sox3 positive neuronal precursor cells are
located in the ventricular zone. F: DAPI staining of the same section
shown in E. G: Merged images from E and F. Notice the enhanced
overall staining of Sox3, including in the more ventral regions of the
ventricular zone, denoted by white arrowheads (compare with the control
embryo shown in B). H–J: More posterior section from the forebrain
region of a z-MO injected tadpole. H: Sox3 neuronal precursors. Note
the expanded ventral labeling denoted by arrowheads. I: Corresponding
DAPI staining for H. J: Merge of Sox3 and DAPI stains. K–M: MyT1
staining for differentiated neurons in a control embryo. K: MyT1 positive
differentiated neurons. Note expanded dorsal area devoid of MyT1
expression within the ventricular zone (denoted by white arrowheads).
L: DAPI staining of the same section shown in K. M: Merged images
from K and L. N–P: Representative sections from a z-MO injected tadpoles.
N: Wide distribution of MyT1 positive differentiated neurons.
Note uniform MyT1 staining in the dorsal ventricular zone (compare
with K). O: DAPI staining of the same section shown in N. P: Merged
images from N and O. fp, floor plate; mz, marginal zone; rp, roof plate;
vn, ventricle. Scale bar = 40 mm in P. |
