| |
Fig. 5. Effects of Mustn1 depletion on Sox9. Lateral views of embryos subjected to in situ whole mount hybridization to detect Sox9 expression. These embryos were from among those scored for phenotypes in Fig. 3 and Fig. 4. High magnification micrographs were taken of lateral views of the head illustrating Sox9 expression at stage 40 in a Co-MO-injected embryo (A), a Mustn1-MO-injected embryo (B) and a Rescue-injected embryo (C). qPCR analysis of Sox9 expression in dorsal tissue dissections of Co-MO-, Mustn1-MO-, and Rescue-injected embryos assessed at stages 25, 34, 39 (graph). No significant difference in Mustn1 mRNA expression was found between Co-MO-injected and Rescue-injected groups at any developmental stage, but a significant difference between Co-MO-injected and Mustn1-MO-injected embryos was detected at stages 34 and 39. All bars represent average values of pooled mRNA (n = 5 embryos). Error bars indicate standard deviation of PCR runs (n = 3). Significance was determined by Mann–Whitney test vs. Co-MO-injected levels, ∗p < .05. Black arrows indicate staining in cranial cartilage, white arrows indicate pharyngeal arch staining, and black arrowheads denote staining of the anterior structures such as mandibular and nasal processes. Scale bar = 250 μm. |
