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FIGURE 2. Overview of the different stages of Xenopus NC development. Embryos were analyzed for pax3 (Stage 14), snail2(Stage 18), twist (Stage 26), and sox9 (Stage 30) expression by in situ hybridization. Nieuwkoop and Faber stages of the analyzed embryos are indicated in brackets. Arrowheads and brackets indicate mandibular (m), hyoid (h), and branchial (b) neural crest streams. NPB: neural plate border. Collagen Type II expression was analyzed by immunostaining at stage 44 to visualize cranial cartilage structures. Cb, ceratobranchial; Ch, ceratohyal; Ir, infrarostral; Me, Meckel's cartilage; Qu, quadrate |
