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Fig. 7. Xapelin and Xmsr are required for the expression of cardiovascular genes. (A) Expressions of XlFli, SCL, and Nkx2.5 in the controlMO (40 ng)-injected embryos at stage 17. (D) Expressions of XlFli, SCL, and Nkx2.5 in the XapelinMO (40 ng)-injected embryos at stage 17. (G) Expressions of XlFli, SCL, and Nkx2.5 in the XmsrMO (20 ng)-injected embryos at stage 17. (J) Expressions of Tie2, α-globin, XPOX2, cTnI, and Nkx2.5 in the controlMO-injected embryos. (O) Expressions of Tie2, α-globin, XPOX2, cTnI, and Nkx2.5 in the XapelinMO-injected embryos. (T) Expressions of Tie2, α-globin, XPOX2, cTnI, and Nkx2.5 in the XmsrMO-injected embryos. (a) Section through the heart region of controlMO-, XapelinMO-, and XmsrMO-injected embryos at stage 30. The black line in panels N, S, and X indicates the level of the sections shown in panels a. Arrow: endocardium. Arrowhead: myocardium, Scale bar: 100 μm. |
