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FIG. 3. Local inhibition of cement and hatching gland by retinoic acid. (A) A retinoic acid droplet (arrow) lying posterior to the cement gland
causing local inhibition of the cement gland. (B) Two examples of embryos that had the retinoic acid droplet (arrow) injected anterior to the
cement gland. In both cases the entire middle of the cement gland was inhibited, resulting in two patches of cement gland on either side of the
droplet. Scale bar, 200 ,..m. (C) Hatching gland, visualized under epifluorescence, was strongly inhibited when in close proximity to the retinoic
acid droplet. The droplet, not visible in this view, was on the left side, where there are fewer, more dispersed cells in the Y pattern. Note that the
overall pattern is still symmetric. Anterior is up. (D} The injected droplet(i) can be visuali-z.ed on the left side of the embryo ~cause of the Oil
fluorescence). The hatching gland (h) is completely absent on the left side of the embryo. Anterior is up. Scale bar, 100 um. |
