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Fig. 3. Microphotographs showing the distribution of XT/34 immunoreactivity (XTI) in tail bud embryos. A: a whole mount embryo (stage 26:
lateral view) stained with pre-immune antiserum for control. B: a whole mount embryo (stage 26) stained with the anti-XT/34 antibody. The XTI
was seen in the trigeminal nerve (arrow). C: a whole mount embryo (stage 34) stained with the anti-XT/34 antibody. Arrow indicates cranial
nerves VII/VIII. Open arrow head indicates the pineal body and the solid arrow heads indicate the XTI located on Rohon-Beard cells and
extramedullary cells along the spinal cord. D: a cross-section of a stage-26 embryo. The XTI was seen in ganglion cells of the trigeminal nerve.
E-H: Cross sections of a stage-34 embryo. E: an intense XTI observed in ganglion cells of the trigeminal nerve (arrows). F: the XTI observed in
the ganglions of cranial nerves VII/VIII (arrow head) and their proximal axons entering the lateral tract of the rhombencephalon (arrow): G: an
intense XTI localized at the pineal body. H: an intense XTI seen in Rohon-Beard cells and extramedullary cells (arrow). M, myotome; No,
notochord; Nt, neural tube; O, otic vesicle; R, retina. Scale bars, in A-C, 1 mm; in D-H, 100 ~m. |
